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Expression of RT6 on activated rat T cells
1Klinik Für Abdominal- Und Transplantationschirurgie, Medizinische Hochschule Hannover, Germany.
Advances in Experimental Medicine and Biology
|January 1, 1997
Summary
T cell activation does not always cause a loss of RT6 expression. Some activated T cells, including CD4+ and CD8+ T cells, remain RT6 positive, suggesting subsets may have distinct roles.
Area of Science:
- Immunology
- Cell Biology
Background:
- RT6 system gene products are typically found on resting peripheral T cells.
- Previous studies suggested T cell activation leads to a loss of RT6 expression.
Purpose of the Study:
- To investigate the expression of RT6 on T cells activated in vitro.
- To determine if T cell activation universally causes RT6 expression loss.
Main Methods:
- In vitro activation of T cell populations using ConA or allogeneic stimulator cells.
- Analysis of RT6 expression on activated T cells over several weeks.
- Co-expression analysis of CD25 and assessment of cell morphology.
Main Results:
- Initial increase in RT6 expression on most activated T cells within two days.
- Subsequent loss of RT6 expression in a subset of activated cells.
- Significant populations of both CD4+ and CD8+ T cells remained RT6 positive throughout the culture period.
Conclusions:
- T cell activation leads to RT6 expression loss only in specific subsets of CD4+ and CD8+ T cells.
- The survival of RT6-negative blasts may explain previous findings, rather than obligatory RT6 loss.
- RT6-CD4+ T cells might represent inflammatory Th1 cells, while RT6+CD4+ T lymphoblasts could be regulatory Th2 cells.