[In vitro analysis for cellular toxicity of polychlorinated biphenys (PCBs) on HeLa cellular proliferation (IV)--the effect of Kanpo preparations on cellular toxicity]

In this study, we investigated the cell toxicity of polychlorinated biphenys (PCBs) as an indicator of the quantity of cellular protein in HeLa cells. Assay for protein concentration was performed by Lowry's method using a commercially available bovine serum albumin as standard preparation. The reductive action of inchinkou-tou, inchinkou, sansisi and daiou on the PCBs toxicity was investigated. The relationship of cell number (0-40.000) to protein concentrations (0-80 micrograms) was shown R2 = 0.993 (Y = 5.0 x 10(-6) X +0.027). The final concentration of these drugs and PCBs was used for 100 micrograms/ml, respectively. The cellar protein concentration increased to 20% with the addition of inchinkou-tou. Inchinkou and sansisi were revealed slightly elevation, but daiou was not. These results suggested that inchinkou-tou, kanpou preparation, was better effective than constructive crude drugs, but statistical evidence could not find.