Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Le(X) structure enhances myocardial differentiation from embryonic stem cells

A Sudou1, H Muramatsu, T Kaname

  • 1Department of Biochemistry, Nagoya University School of Medicine, Japan.

Cell Structure and Function
|April 1, 1997
PubMed
Summary

Alpha-1,3-fucosyltransferase (Fuc T IV) enhances embryonic stem cell differentiation into myocardial cells. Lewis X structures promote enhanced myocardial cell survival and differentiation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Λ[over ¯]-Λ Baryon-Number-Violating Oscillations in the Decay J/ψ→pK^{-}Λ[over ¯]+c.c.

Physical review letters·2023
Same author

Observation of an Isoscalar Resonance with Exotic J^{PC}=1^{-+} Quantum Numbers in J/ψ→γηη^{'}.

Physical review letters·2022
Same author

Observation of an a_{0}-like State with Mass of 1.817 GeV in the Study of D_{s}^{+}→K_{S}^{0}K^{+}π^{0} Decays.

Physical review letters·2022
Same author

First Observation of the Direct Production of the χ_{c1} in e^{+}e^{-} Annihilation.

Physical review letters·2022
Same author

Observation of Resonance Structures in e^{+}e^{-}→π^{+}π^{-}ψ_{2}(3823) and Mass Measurement of ψ_{2}(3823).

Physical review letters·2022
Same author

Observation of a State X(2600) in the π^{+}π^{-}η' System in the Process J/ψ→γπ^{+}π^{-}η'.

Physical review letters·2022

Area of Science:

  • Biochemistry
  • Developmental Biology
  • Stem Cell Research

Background:

  • Embryonic stem cells (ESCs) are crucial for studying cell differentiation.
  • Myocardial differentiation is a complex process with therapeutic potential.
  • The role of specific enzymes like alpha-1,3-fucosyltransferase (Fuc T IV) in differentiation is not fully understood.

Purpose of the Study:

  • To investigate the effect of alpha-1,3-fucosyltransferase (Fuc T IV) on the differentiation of embryonic stem cells into myocardial cells.
  • To determine if the Lewis X structure, a product of Fuc T IV, influences myocardial cell differentiation and survival.

Main Methods:

  • Transfection of embryonic stem cells with alpha-1,3-fucosyltransferase (Fuc T IV) cDNA under a beta-actin/cytomegalovirus enhancer/promoter.

Related Experiment Videos

  • Culturing and differentiation of transfected (positive) and non-transfected (negative) cell clones.
  • Comparison of differentiation efficiency and cell survival rates between positive, negative, and parental cells.

    • Main Results:

    • ESC clones with integrated Fuc T IV cDNA exhibited significantly enhanced differentiation into myocardial cells compared to controls.
    • Myocardial cells derived from Fuc T IV-transfected clones demonstrated improved survival rates.
    • The Lewis X structure, produced by Fuc T IV, is implicated in promoting myocardial differentiation.

    Conclusions:

    • Alpha-1,3-fucosyltransferase (Fuc T IV) expression enhances myocardial differentiation from ESCs.
    • The Lewis X structure plays a key role in improving myocardial cell differentiation and survival.
    • Integrin action may be involved in the mechanism underlying Fuc T IV-mediated enhancement of myocardial differentiation.