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Neurotensin increases intestinal adaptation and reduces enteroglucagon-like immunoreactivity after large bowel
A Mata1, I A Gómez de Segura, C Largo
1Service of Experimental Surgery, Hospital La Paz, Madrid, Spain.
Objective:
To assess the effects of giving neurotensin on intestinal adaptation after colectomy and their relation to enteroglucagon-like immunoreactivity.
Design:
Laboratory experiment.
Setting:
Teaching hospital, Spain.
Material:
55 Male Wistar rats.
Interventions:
All animals were anaesthetised before undergoing laparotomy; 24 animals had 75% of their colon resected. Half of the animals (12 in each group) were treated with neurotensin (600 micrograms/kg body wt/day) for 14 days.
Main Outcome Measures:
Differences in the number of mitoses and in nuclear antigen staining of proliferating cells in the intestinal mucosal crypts; plasma enteroglucagon-like immunoreactivity.
Results:
After colon resection, the proliferative status, number of mitoses (p < 0.01), and nuclear antigen staining of proliferating cells (p < 0.001) increased significantly in the jejunum of animals treated with neurotensin (p < 0.05). Less pronounced effects were observed in colon and ileum. Plasma enteroglucagon-like immunoreactivity levels fell significantly in all animals given neurotensin (p < 0.05).
Conclusions:
Neurotensin increases the adaptive intestinal process after colon resection and reduces plasma enteroglucagon-like immunoreactivity in rats.