Related Experiment Video
Updated: Aug 8, 2026

In vitro Organoid Culture of Primary Mouse Colon Tumors
Published on: May 17, 2013
The adenomatous polyposis coli (APC) tumor suppressor
1Onyx Pharmaceuticals Inc., Richmond, CA 94806, USA. paul@onyx-pharm.com
Abstract:
Defects in the APC gene are inarguably linked to the progression of colon cancers that arise both sporadically and through the transmission of germline mutations. Genetic evidence from humans and mouse models suggest that APC is a classic tumor suppressor in that both alleles likely require inactivation for tumor growth to ensue. Nearly all of the mutations, germline and somatic, result in premature termination of the single polypeptide chain, normally consisting of 2843 amino acids. Several definable motifs have now been mapped to the linear amino acid sequence of the APC polypeptide. These include an oligomerization domain, armadillo repeats, binding sites for beta-catenin, the human discs large protein, microtubules, and other proteins of unknown function. Inactivation of APC in cancer is likely due to loss of function(s) normally associated with the deleted protein structure.
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