Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basic aminopeptidase from rabbit kidney: purification and partial characterization

S M Oliveira1, J O Freitas Júnior, K B Alves

  • 1Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brasil.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|November 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracranial compliance in type 2 diabetes mellitus and its relationship with the cardiovascular autonomic nervous control.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2022
Same author

High-Frequency Acceleration: Therapeutic Tool to Preserve Bone following Tooth Extractions.

Journal of dental research·2015
Same author

What do we know about the α/β for prostate cancer?

Medical physics·2012
Same author

Osteogenic effect of high-frequency acceleration on alveolar bone.

Journal of dental research·2012
Same author

Performance and nematode infection of ewe lambs on intensive rotational grazing with two different cultivars of Panicum maximum.

Tropical animal health and production·2007
Same author

Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy.

Memorias do Instituto Oswaldo Cruz·2005
Same journal

RETRACTION: NOP14 inhibits melanoma proliferation and metastasis by regulating Wnt/β-catenin signaling pathway.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

RETRACTION: Differential expression of microRNA-411 and 376c is associated with hypertension in pregnancy.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

RETRACTION: Comparison of two treatments for dry eye disease after corneal refractive surgery.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

The emerging role of the microbiome in bladder cancer: prognostic implications and treatment response.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

Standardizing the lateral fluid percussion model of trauma in marmosets (Callithrix jacchus): regional vulnerability and inflammatory response.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

COVID-19 mortality risk among women with ovarian cancer: a matched case-control study.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
See all related articles

Rabbit kidney homogenates contain aminopeptidase activity. A specific 78 kDa enzyme, highly active with Arg-NA, was isolated and characterized, showing sensitivity to inhibitors.

Area of Science:

  • Biochemistry
  • Enzymology
  • Proteomics

Background:

  • Aminopeptidases are crucial enzymes involved in protein metabolism.
  • Rabbit kidney is a rich source of various enzymatic activities.
  • Understanding specific aminopeptidases aids in elucidating physiological and pathological processes.

Purpose of the Study:

  • To isolate and characterize a specific aminopeptidase from rabbit kidney.
  • To determine the kinetic properties and substrate specificity of the isolated enzyme.
  • To investigate the enzyme's sensitivity to various inhibitors.

Main Methods:

  • Rabbit kidney homogenate preparation with Triton X-100.
  • Ion-exchange chromatography for initial separation of aminopeptidase activity.

Related Experiment Videos

  • Superdex 75 gel filtration chromatography for further purification.
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for molecular mass determination.
  • Enzyme kinetics assays using L-aminoacyl-2-naphthylamides (AA-NA) and determination of inhibition constants.

    • Main Results:

    • Four distinct peaks of aminopeptidase activity were identified after ion-exchange chromatography.
    • A single protein band of approximately 78 kDa was observed for the purified enzyme.
    • The enzyme exhibited highest activity and Vmax/KM ratio with Arg-NA.
    • Activity was significantly modulated by NaCl, and inhibited by sodium p-hydroxymercuribenzoate and o-phenanthroline.
    • Puromycin and bestatin acted as competitive inhibitors.

    Conclusions:

    • A novel 78 kDa aminopeptidase from rabbit kidney was successfully isolated and purified.
    • The enzyme demonstrates a preference for Arg-NA and is sensitive to specific inhibitors, suggesting its unique role.
    • Further studies on this enzyme could reveal its physiological significance in renal function and disease.