Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microgravity tissue engineering

L E Freed1, G Vunjak-Novakovic

  • 1Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge 02139, USA.

In Vitro Cellular & Developmental Biology. Animal
|May 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vascularized Tissue-Engineered Model for Studying Drug Resistance in Neuroblastoma.

Theranostics·2017
Same author

Biomechanical regulation of drug sensitivity in an engineered model of human tumor.

Biomaterials·2017
Same author

Mimicking biophysical stimuli within bone tumor microenvironment.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2016
Same author

Passage-dependent relationship between mesenchymal stem cell mobilization and chondrogenic potential.

Osteoarthritis and cartilage·2014
Same author

Cartilage constructs engineered from chondrocytes overexpressing IGF-I improve the repair of osteochondral defects in a rabbit model.

European cells & materials·2013
Same author

Spatial regulation of human mesenchymal stem cell differentiation in engineered osteochondral constructs: effects of pre-differentiation, soluble factors and medium perfusion.

Osteoarthritis and cartilage·2010
Same journal

Establishment and characterization of an ovarian cell line from red seabream (Pagrus major) and its application in fish toxicology.

In vitro cellular & developmental biology. Animal·2026
Same journal

DIRAS2 promotes the progression of oral leukoplakia via suppressing ferroptosis.

In vitro cellular & developmental biology. Animal·2026
Same journal

Distinct adhesion energy and extracellular matrix profiles of healthy and osteoarthritic human chondrocytes.

In vitro cellular & developmental biology. Animal·2026
Same journal

Pesticides-induced cellular toxicity in the spinal cord cell line of Lates calcarifer.

In vitro cellular & developmental biology. Animal·2026
Same journal

Serotype-specific effects of adenovirus 5 and 52 E4ORF1 proteins on endothelial cell-mediated regulation of hematopoietic stem cell expansion and lineage differentiation.

In vitro cellular & developmental biology. Animal·2026
Same journal

ALKBH4 confers ferroptosis resistance and drives tumorigenesis via dysregulation of GPX4 in breast cancer cells.

In vitro cellular & developmental biology. Animal·2026
See all related articles

Simulated microgravity in rotating bioreactors enhances tissue engineering. Cartilage and cardiac tissue constructs showed improved formation and composition, particularly higher glycosaminoglycan (GAG) content in cartilage.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cell Biology

Background:

  • Tissue engineering aims to regenerate tissues using cells, scaffolds, and bioreactors.
  • Simulated microgravity offers a unique environment for cell culture and tissue development.

Purpose of the Study:

  • To investigate the feasibility of tissue engineering under simulated microgravity.
  • To compare the effects of simulated microgravity with other culture conditions on tissue formation.

Main Methods:

  • Utilized rotating bioreactors to create simulated microgravity for cell-polymer constructs.
  • Cultivated cartilage and cardiac cells on 3D scaffolds in free-fall conditions.
  • Compared construct composition under simulated microgravity, solid body rotation, turbulent mixing, and orbital mixing.

Related Experiment Videos

Main Results:

  • Demonstrated successful engineering of cartilaginous and cardiac tissues.
  • Simulated microgravity promoted formation of cartilaginous constructs with high GAG content.
  • Cardiac constructs exhibited spontaneous and synchronous contractions.

Conclusions:

  • Simulated microgravity is a feasible and beneficial environment for tissue engineering.
  • This method enhances the quality and composition of engineered cartilage and cardiac tissues.
  • Optimized GAG content in cartilage constructs is crucial for load-bearing applications.