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Isolation and partial characterization of a fatty-acid-binding protein from Ascaris suum reproductive tissue
A M Timanova1, T Marti, R D Walter
1Institute of Experimental Pathology and Parasitology, Bulgarian Academy of Sciences, Sofia.
Parasitology Research
|January 1, 1997
Abstract:
A 12-kDa fatty-acid-binding protein was purified to homogeneity from Ascaris suum reproductive tissue as confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino-acid-sequence analysis of the protein revealed its identity with the ABA-1 allergen protein isolated from A. suum pseudocoelomic fluid. Fatty-acid binding by the protein from A. suum reproductive tissue was investigated using the Lipidex 1000 assay, which revealed the presence of a single class of fatty-acid-binding sites with an apparent dissociation constant for palmitate of about 0.8 microM.