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Flow cytometric determination of binding of monoclonal antibodies to human spermatozoa
F Schwarzinger1, T Schüller, W Krause
1Department of Andrology, Faculty of Medicine, Philipps-Universität, Marburg, Germany.
Although several monoclonal antibodies to sperm antigens are available, only few systematical reports on the appearance of distinct antibody binding structures in spermatozoa of infertile patients are available. We studied the binding frequency of six monoclonal antibodies (TUS-1, -2, -10, -17, -19, -20) by means of a flow cytometer in different semen samples and their possible relationship to other sperm parameters, in particular to the motility parameters as determined by computer-assisted semen analysis. The percentage of vital spermatozoa binding the different antibodies was 21.5% (TUS-2) to 52.1% (TUS-20). The percentage binding was higher in samples with normozoospermia than in those with oligo-asthenoteratozoospermia. A significant correlation occurred between sperm concentration and the percentage of spermatozoa stained by TUS-2 and TUS-17; between motile spermatozoa and those binding TUS-17 and TUS-20; the velocity average path and those binding TUS-17; an inverse correlation occurred between the morphologically-abnormal spermatozoa and those binding TUS-20; an inverse correlation between TUS-17 and acrosin. We conclude from our results that although the antigens of the antibodies concerned are not yet characterized, the determination of antibody binding will give additional information on the functional status during capacitation and acrosome reaction of spermatozoa.
Although several monoclonal antibodies to sperm antigens are available, only few systematical reports on the appearance of distinct antibody binding structures in spermatozoa of infertile patients are available. We studied the binding frequency of six monoclonal antibodies (TUS-1, -2, -10, -17, -19, -20) by means of a flow cytometer in different semen samples and their possible relationship to other sperm parameters, in particular to the motility parameters as determined by computer-assisted semen analysis. The percentage of vital spermatozoa binding the different antibodies was 21.5% (TUS-2) to 52.1% (TUS-20). The percentage binding was higher in samples with normozoospermia than in those with oligo-asthenoteratozoospermia. A significant correlation occurred between sperm concentration and the percentage of spermatozoa stained by TUS-2 and TUS-17; between motile spermatozoa and those binding TUS-17 and TUS-20; the velocity average path and those binding TUS-17; an inverse correlation occurred between the morphologically-abnormal spermatozoa and those binding TUS-20; an inverse correlation between TUS-17 and acrosin. We conclude from our results that although the antigens of the antibodies concerned are not yet characterized, the determination of antibody binding will give additional information on the functional status during capacitation and acrosome reaction of spermatozoa.