Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Luminal bacteria and small-intestinal permeability

S M Riordan1, C J McIver, D H Thomas

  • 1Dept. of Gastroenterology, Prince of Wales Hospital, Sydney, Australia.

Scandinavian Journal of Gastroenterology
|June 1, 1997
PubMed
Summary

Small intestinal bacterial overgrowth (SIBO) with colonic-type bacteria increases small-intestinal permeability, independent of age or vitamin B12 levels. This functional disturbance is often missed by standard histology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The clustering of Cardiovascular, Renal, Adipo-Metabolic Eye and Liver disease with type 2 diabetes.

Metabolism: clinical and experimental·2021
Same author

The Heart Failure with Preserved Ejection Fraction Conundrum-Redefining the Problem and Finding Common Ground?

Current heart failure reports·2020
Same author

Longitudinal monitoring of anti-saliva antibodies as markers of repellent efficacy against Phlebotomus perniciosus and Phlebotomus papatasi in dogs.

Medical and veterinary entomology·2018
Same author

The effect of resonance on transient microbubble acoustic response: Experimental observations and numerical simulations.

The Journal of the Acoustical Society of America·2018
Same author

A prospective gating method to acquire a diverse set of free-breathing CT images for model-based 4DCT.

Physics in medicine and biology·2018
Same author

Development of a novel multiplexed qPCR and Pyrosequencing method for the detection of human pathogenic yersiniae.

International journal of food microbiology·2017

Area of Science:

  • Gastroenterology
  • Microbiology
  • Human Physiology

Background:

  • The impact of gut bacteria on small intestinal permeability is not fully understood.
  • This study investigates the relationship between small intestinal bacterial overgrowth (SIBO) and small intestinal permeability.

Purpose of the Study:

  • To assess the influence of luminal bacteria on small-intestinal permeability.
  • To investigate the role of SIBO with colonic-type flora in altering small intestinal barrier function.

Main Methods:

  • Investigated 34 subjects for SIBO using small intestinal aspirate culture.
  • Performed lactulose/mannitol small intestinal permeability tests and assessed histology.
  • Measured serum vitamin B12 and compared permeability in SIBO patients versus 34 controls.

Related Experiment Videos

Main Results:

  • Increased urinary lactulose/mannitol ratios observed in SIBO patients with colonic-type flora (P < 0.0005).
  • Elevated lactulose levels, not reduced mannitol, drove the increased permeability.
  • Increased CD8+ intraepithelial lymphocytes noted, but not directly correlated with permeability severity.

Conclusions:

  • Small intestinal permeability is elevated in SIBO with colonic-type bacteria, irrespective of age or vitamin B12 status.
  • Increased CD8+ lymphocytes are present but unlikely to be the primary cause of permeability changes.
  • Standard light microscopy may underestimate functional small intestinal disturbances in SIBO.