Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

V(D)J recombination moves in vitro

D G Schatz1

  • 1Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520-8011, USA.

Seminars in Immunology
|June 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biochemistry of V(D)J recombination.

Current topics in microbiology and immunology·2006
Same author

Identification of basic residues in RAG2 critical for DNA binding by the RAG1-RAG2 complex.

Molecular cell·2001
Same author

Location, location, location: the cell biology of immunoglobulin allelic control.

Nature immunology·2001
Same author

Factors and forces controlling V(D)J recombination.

Advances in immunology·2001
Same author

Cell-cycle-regulated DNA double-stranded breaks in somatic hypermutation of immunoglobulin genes.

Nature·2000
Same author

Genetic modulation of T cell receptor gene segment usage during somatic recombination.

The Journal of experimental medicine·2000

Recent advancements reveal that the RAG1 and RAG2 proteins are essential for V(D)J recombination DNA cleavage. These proteins, along with the heptamer and nonamer sequences, precisely guide the DNA nicking and strand transfer processes.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • V(D)J recombination is a crucial process for adaptive immune system development.
  • Understanding the molecular mechanisms of V(D)J recombination is vital for comprehending immune diversity.

Purpose of the Study:

  • To elucidate the roles of RAG1 and RAG2 proteins in the DNA cleavage phase of V(D)J recombination.
  • To investigate the significance of recombination signal sequences (heptamer and nonamer) in V(D)J recombination.

Main Methods:

  • Development and utilization of in-vitro systems for studying V(D)J recombination.
  • Analysis of DNA cleavage, nicking, and strand transfer steps.
  • Investigation of protein-DNA interactions between RAG proteins and recombination signal sequences.

Related Experiment Videos

Main Results:

  • RAG1 and RAG2 proteins are directly involved in DNA recognition and cleavage.
  • Both RAG1 and RAG2 are necessary for nicking and strand transfer.
  • The heptamer sequence is critical for precise cleavage targeting.
  • The nonamer sequence is important for initial DNA binding and is contacted by RAG1.

Conclusions:

  • The RAG1 and RAG2 proteins are the key enzymatic players in V(D)J recombination cleavage.
  • Recombination signal sequences, particularly the heptamer and nonamer, play distinct but essential roles in guiding the reaction.
  • Established in-vitro systems facilitate continued rapid progress in understanding V(D)J recombination.