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Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Cardiovascular effects of nitric oxide in the rostral ventrolateral medulla of rats
S Kagiyama1, T Tsuchihashi, I Abe
1Second Department of Internal Medicine, Faculty of Medicine, Kyushu University, Higashi-ku, Fukuoka, Japan. kagiyama@intmed2.med.kyushu-u.ac.jp
Abstract:
To investigate the cardiovascular role of nitric oxide (NO) in the rostral ventrolateral medulla (RVLM), NOC 18, an NO donor, was microinjected into the RVLM of rats. NOC 18 significantly decreased mean arterial pressure (MAP). Pre-treatment with an NO trapper, carboxy-PTIO, abolished the NOC 18-induced decrease in MAP. Microinjection of L-NAME, an NO synthase inhibitor, increased MAP. L-Arginine reduced MAP and inhibited the pressor response induced by L-NAME. Results suggest that NO acts on the RVLM neurons and plays an important role in cardiovascular regulation.
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