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Related Experiment Videos

Improved human islet isolation using a new enzyme blend, liberase

E Linetsky1, R Bottino, R Lehmann

  • 1Diabetes Research Institute, University of Miami School of Medicine, and the Miami Veterans Administration Medical Center, Florida 33136, USA.

Diabetes
|July 1, 1997
PubMed
Summary

Liberase, a new enzyme mixture, significantly improves islet isolation yield and quality compared to traditional collagenase. This standardized enzyme offers better reproducibility and gentler tissue processing for human islet isolation.

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Area of Science:

  • Endocrinology
  • Biochemistry
  • Surgical Research

Background:

  • Enzymatic digestion of pancreases is crucial for islet isolation.
  • Variability in collagenase enzyme activity hinders reproducible islet isolation.

Purpose of the Study:

  • To compare the effectiveness of Liberase, a standardized enzyme mixture, against traditional collagenase (type P) for human islet isolation.
  • To evaluate the impact of Liberase on islet yield, quality, and function.

Main Methods:

  • Compared 50 islet isolations using Liberase with 36 using collagenase type P.
  • Analyzed parameters including islet number, yield per gram, and islet equivalents.
  • Assessed islet size, fragmentation, and in vitro/in vivo function.

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Main Results:

  • Liberase significantly increased islet yield and all measured yield parameters.
  • Islets isolated with Liberase were larger, less fragmented, and showed better anatomical integrity.
  • No significant differences in donor age, ischemia time, or digestion time were noted.
  • Functional profiles of islets isolated with either enzyme were similar.

Conclusions:

  • Liberase offers improved reproducibility and higher islet yield compared to collagenase.
  • The standardized enzyme provides gentler enzymatic action, enhancing islet quality.
  • Liberase represents a valuable tool for advancing human islet isolation techniques.