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Related Experiment Videos

Ran1 functions to control the Cdc10/Sct1 complex through Puc1

M Caligiuri1, T Connolly, D Beach

  • 1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724, USA.

Molecular Biology of the Cell
|June 1, 1997
PubMed
Summary
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The Ran1 protein kinase regulates cell cycle commitment in fission yeast by controlling the association of Cdc10 and Puc1. This mechanism impacts cell fate decisions during the G1/S-phase transition.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The G1/S-phase transition and cell cycle commitment are critical regulatory points in eukaryotic cell division.
  • Fission yeast (Schizosaccharomyces pombe) serves as a model organism for studying cell cycle control.
  • Key regulators include the Cdc2 protein kinase and the Sct1/Cdc10 transcription complex.

Purpose of the Study:

  • To biochemically analyze the regulation of the G1/S-phase transition in Schizosaccharomyces pombe.
  • To elucidate the role of the Ran1 protein kinase in cell cycle commitment and cell fate.
  • To investigate the interaction between Ran1, Cdc10, and Puc1.

Main Methods:

  • Biochemical analysis of protein interactions and kinase activity.
  • In vivo association studies of Puc1, Ran1, and Cdc10.

Related Experiment Videos

  • Assessment of protein phosphorylation states.

    • Main Results:

    • Ran1 kinase activity is required for Sct1/Cdc10 complex formation, in addition to Cdc2.
    • Puc1 cyclin associates with Ran1 and Cdc10 in vivo.
    • Ran1 controls the association between Puc1 and Cdc10, and its inactivation alters Cdc10 phosphorylation.
    • Ran1 inactivation promotes meiotic pathway activation under inhibitory conditions.

    Conclusions:

    • Ran1 protein kinase regulates cell fate by controlling the Cdc10 and Puc1 interaction during the G1/S-phase transition.
    • This study provides biochemical evidence for a novel mechanism of cell cycle control in fission yeast.
    • Understanding these pathways is crucial for comprehending cell division regulation and differentiation.