Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extracellular biotransformation potential in mouse airways

A Clouter1, R J Richards

  • 1MRC Toxicology Unit, Leicester University, U.K.

The International Journal of Biochemistry & Cell Biology
|March 1, 1997
PubMed
Summary

Freshly isolated mouse Clara cells and lung lavage fluid exhibit significant biotransformation potential. This suggests that xenobiotic metabolism can occur extracellularly in the lung airways, involving both phase 1 and phase 2 reactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigating the Debate: Does DHEA Alter Food Intake?

Nutritional neuroscience·2016
Same author

Conventional and toxicogenomic assessment of the acute pulmonary damage induced by the instillation of Cardiff PM10 into the rat lung.

The Science of the total environment·2006
Same author

Regarding the use of HOMA to aid drug selection.

Diabetic medicine : a journal of the British Diabetic Association·2005
Same author

Serum exposed to nanoparticle carbon black displays increased potential to induce macrophage migration.

Toxicology letters·2005
Same author

Increased inflammation and altered macrophage chemotactic responses caused by two ultrafine particle types.

Occupational and environmental medicine·2004
Same author

The spatial and temporal variations in PM10 mass from six UK homes.

The Science of the total environment·2004

Area of Science:

  • Pulmonary Toxicology
  • Xenobiotic Metabolism
  • Cell Biology

Background:

  • Clara cells are crucial for lung defense and xenobiotic metabolism.
  • The extracellular lining fluid's role in lung biotransformation is not fully understood.
  • Investigating metabolic potential in isolated cells and lavage fluid is key to understanding lung detoxification.

Purpose of the Study:

  • To determine if isolated mouse bronchiolar Clara cells retain biotransformation capabilities.
  • To investigate the presence of phase 1 and phase 2 metabolic components in lung airway extracellular lining fluid.

Main Methods:

  • Isolation of Clara cells and collection of acellular lung lavage fluid from perfused mouse lungs.
  • Assay of ethoxycoumarin-O-deethylase, NADPH-dependent cytochrome c reductase, and glutathione-S-transferase activities.

Related Experiment Videos

  • Quantification of non-protein sulphydryls (reduced glutathione) in both isolated cells and lavage fluid.

    • Main Results:

    • Isolated Clara cells demonstrated high levels of all measured metabolic enzyme activities and glutathione.
    • Acellular lavage fluid also contained significant mono-oxygenase, reductase, and transferase activities.
    • High non-protein sulphydryl levels were detected in the extracellular lining fluid, indicating metabolic capacity.

    Conclusions:

    • Freshly isolated Clara cells maintain their functional biotransformation potential.
    • The lung's extracellular lining fluid possesses components necessary for both phase 1 and phase 2 xenobiotic metabolism.
    • Apocrine secretion by Clara cells may contribute to the observed extracellular biotransformation capacity in lung airways.