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Effect of oxygen on relaxation of retinal pericytes by sodium nitroprusside
I O Haefliger1, Q Chen, D R Anderson
1Laboratory for Ocular Pharmacology and Physiology, University Eye Clinic Basel, Switzerland.
Background:
This study addresses whether oxygen modulates the relaxation induced in retinal pericytes by sodium nitroprusside (SNP), a nitric oxide (NO) donor that stimulates the NO/guanylate cyclase pathway.
Methods:
Bovine retinal pericytes were cultured on silicone. On the silicone surface, basal pericyte contractile tone induces wrinkles. Drug-induced changes in pericyte contractile tone were assessed by changes in the number of wrinkles. The effects of 100% nitrogen (hypoxia) and 100% oxygen (hyperoxia) were studied on: (a) the basal tone of quiescent pericytes, (b) the relaxation to 3 and 10 microM SNP or 1 microM forskolin, and (c) the recontraction that followed the washout of 3 microM SNP or 1 microM forskolin.
Results:
Neither hypoxia nor hyperoxia had any apparent influence on pericyte basal tone, on forskolin-induced relaxation, or on pericyte recontraction after a forskolin-induced relaxation. In hypoxia, relaxations to SNP 3 microM (P < 0.05) and 10 microM (P < 0.01) were significantly more pronounced than in hyperoxia. Hypoxia also reduced the recontraction after an SNP-induced relaxation (P < 0.001).
Conclusion:
Oxygen modulates the relaxation of bovine retinal pericytes evoked by SNP (guanylate cyclase-mediated), but not the relaxation induced by forskolin (adenylate cyclase-mediated). These results suggest that in the retinal capillary circulation an interaction between oxygen and the NO/guanylate cyclase pathway modulates pericyte tone, and thus potentially blood flow.