Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two-dimensional crystals of apoferritin

H Yoshimura1

  • 1Department of Physics, School of Science and Technology, Meiji University, Kawasaki, Japan.

Advances in Biophysics
|January 1, 1997
PubMed
Summary

A novel 2D crystallization method enables protein surface modification and the creation of uniform, fine particles. This technique, using apoferritin, facilitates the development of advanced materials with tunable properties for various applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pulmonary blastoma. Comparison between its epithelial components and fetal bronchial epithelium.

Acta pathologica japonica·1992
Same author

A new metabolite of 2,4,3',4'-tetrachlorobiphenyl in rat feces.

Chemical & pharmaceutical bulletin·1992
Same author

Sex difference in hepatic microsomal aldehyde oxygenase activity in different strains of mice.

Research communications in chemical pathology and pharmacology·1992
Same author

Tissue-specific expression, induction, and inhibition through metabolic intermediate-complex formation of guinea pig cytochrome P450 belonging to the CYP2B subfamily.

Archives of biochemistry and biophysics·1992
Same author

[Metabolite of 15-p-iodophenyl-3(R,S)-pentadecanoic acid (123I) in blood and urine].

Kaku igaku. The Japanese journal of nuclear medicine·1992
Same author

Metabolism of 2,4,5,2',4',5'-hexachlorobiphenyl with liver microsomes of phenobarbital-treated dog; the possible formation of PCB 2,3-arene oxide intermediate.

Xenobiotica; the fate of foreign compounds in biological systems·1992

Area of Science:

  • Materials Science
  • Biotechnology
  • Crystallography

Background:

  • Protein crystallization is crucial for structural biology and materials science.
  • Existing methods for fabricating nanoscale materials are often complex and lack precision.
  • Apoferritin's unique structure offers potential for creating functional nanomaterials.

Purpose of the Study:

  • To develop a simple and versatile 2D crystallization method for protein arrays.
  • To demonstrate the modification of protein crystal surfaces and the formation of layered structures.
  • To explore the application of apoferritin 2D crystals in fabricating uniform fine particles.

Main Methods:

  • Utilizing an unfolded protein film as a support for 2D crystal formation.
  • Injecting chemical reagents into the subphase post-crystallization for surface modification.
  • Employing glutaraldehyde as a cross-linker to stabilize protein crystals.
  • Layering additional proteins onto existing apoferritin arrays using cross-linkers.

Main Results:

  • A straightforward method for creating 2D protein crystals on a supporting film was established.
  • Protein crystal surfaces were successfully modified, and adjacent proteins were cross-linked for stabilization.
  • A second layer of proteins could be assembled on apoferritin arrays.
  • Uniform fine particles with tunable core sizes (e.g., 6 nm) were fabricated using apoferritin 2D crystals.

Conclusions:

  • The described 2D crystallization method offers a versatile platform for protein array fabrication and modification.
  • Apoferritin 2D crystals provide an effective means for producing well-defined nanoparticles with potential for magnetic applications.
  • This approach holds promise for advancing electron crystallography and developing novel functional materials through protein engineering.

Related Experiment Videos