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Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 7, 2009

Automated sample preparation using membrane microtiter extraction for bioanalytical mass spectrometry

J Janiszewski1, P Schneider, K Hoffmaster

  • 1Central Research Division, Pfizer INC., Groton, CT 06340, USA.

Rapid Communications in Mass Spectrometry : RCM
|January 1, 1997
PubMed
Summary

Automated membrane solid phase extraction (SPE) in a 96-well format enables rapid drug analysis in biological fluids. This high-throughput method significantly accelerates sample preparation for liquid chromatography-mass spectrometry, supporting drug discovery.

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Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Biotechnology

Background:

  • Traditional drug analysis in biological fluids is often time-consuming.
  • Current methods like solvent extraction or single cartridge SPE lack high-throughput capabilities.
  • Automated sample preparation is crucial for modern drug discovery workflows.

Purpose of the Study:

  • To develop and apply an automated membrane solid phase extraction (SPE) method for drug analysis in biological fluids.
  • To enhance the speed and efficiency of sample preparation for high-performance liquid chromatography-mass spectrometry (HPLC/MS).
  • To demonstrate the utility of a 96-well membrane SPE format for drug discovery support.

Main Methods:

  • Development of an automated membrane SPE protocol using a modified programmable liquid handling station (Quadra 96).
  • Utilized a novel rapid succession protocol for condition, load, and wash steps to prevent SPE bed drying and improve precision.
  • Employed a 96-well microtiter plate format for simultaneous sample processing.
  • Quantitative analysis of Ziprasidone in plasma as a test case.

Main Results:

  • Achieved automated extraction of 96 samples in 10 minutes, a 30-fold increase in speed compared to manual methods.
  • Enabled direct HPLC injection due to small elution solvent volumes, eliminating solvent evaporation.
  • Demonstrated favorable quantitative results for Ziprasidone analysis compared to traditional methods.
  • Successfully applied the method to analyze numerous drug prototypes in biological fluids.

Conclusions:

  • Automated 96-well membrane SPE offers a significant advancement in sample preparation speed and efficiency for drug analysis.
  • The developed method is compatible with high-throughput HPLC/MS, accelerating drug discovery efforts.
  • This technique provides a robust and rapid solution for analyzing drugs in biological matrices.