Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adenosine A2 receptor function in rat ventricular myocytes

J G Dobson1, R A Fenton

  • 1Department of Physiology, University of Massachusetts Medical School, Worcester, USA..

Cardiovascular Research
|May 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mucin-mediated nanocarrier disassembly for triggered uptake of oligonucleotides as a delivery strategy for the potential treatment of mucosal tumours.

Nanoscale·2015
Same author

The vascular Ca2+-sensing receptor regulates blood vessel tone and blood pressure.

American journal of physiology. Cell physiology·2015
Same author

Vasopressin regulation of sodium transport in the distal nephron and collecting duct.

American journal of physiology. Renal physiology·2015
Same author

Characterization of a novel phosphorylation site in the sodium-chloride cotransporter, NCC.

The Journal of physiology·2012
Same author

Short communication: Effects of dietary nitrogen concentration on messenger RNA expression and protein abundance of urea transporter-B and aquaporins in ruminal papillae from lactating Holstein cows.

Journal of dairy science·2011
Same author

RECENT DISCOVERIES IN THE PATHOLOGY OF THE NASAL AND AURAL MUCOSA.

Canadian Medical Association journal·2010

Stimulating adenosine A2a receptors in rat ventricular myocytes increases contractility through both cAMP-dependent and -independent pathways, indicating their functional significance.

Area of Science:

  • Cardiovascular Physiology
  • Molecular Pharmacology
  • Cell Biology

Background:

  • Adenosine receptors play crucial roles in regulating cardiac function.
  • Understanding the specific roles of adenosine receptor subtypes in ventricular myocytes is essential for cardiovascular research.

Purpose of the Study:

  • To investigate the functional significance of adenosine A2 receptor stimulation in mammalian ventricular myocytes.
  • To elucidate the mechanisms underlying adenosine A2 receptor-mediated effects on cardiac contractility.

Main Methods:

  • Isolated contracting rat ventricular myocytes were used to assess contractile responses.
  • Adenylyl cyclase and cyclic AMP (cAMP) levels were measured following adenosine receptor stimulation.
  • Specific agonists and antagonists for A1 and A2 receptors were employed.

Related Experiment Videos

Main Results:

  • Adenosine A1 receptors were confirmed to inhibit isoproterenol-induced inotropic and adenylyl cyclase responses.
  • Adenosine A2 receptor agonists (CGS-21680, NECA, SHA-082, adenosine) concentration-dependently increased myocyte inotropy.
  • A2 receptor antagonists (CGS-15943, CSC) blocked these contractile effects, while cAMP elevation required higher agonist concentrations.

Conclusions:

  • The study suggests the presence of functional adenosine A2a receptors in rat ventricular myocytes.
  • These receptors increase myocyte inotropy through both cAMP-dependent and -independent mechanisms.
  • Adenosine A2a receptors represent a potential target for modulating cardiac contractility.