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Related Experiment Videos

Growth cone interactions with purified cell and substrate adhesion molecules visualized by interference reflection

J Drazba1, P Liljelund, C Smith

  • 1Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.

Brain Research. Developmental Brain Research
|June 18, 1997
PubMed
Summary

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Growth cone contact with substrates varies by cell adhesion molecule. N-cadherin and L1 promote closer growth cone-substrate interactions, influencing neuronal growth and migration dynamics.

Area of Science:

  • Cell Biology
  • Neuroscience
  • Biophysics

Background:

  • Neuronal growth cone migration is crucial for neural development.
  • Understanding growth cone-substrate interactions is key to neural development research.
  • Limited knowledge exists on how growth cones initially contact substrates and how these contacts evolve during migration.

Purpose of the Study:

  • To investigate the substrate contact patterns of chick retinal ganglion cell growth cones.
  • To analyze how growth cone interactions differ across various cell adhesion molecules (laminin, merosin, N-cadherin, L1) and poly-L-lysine.
  • To correlate substrate contact dynamics with growth cone migration rates.

Main Methods:

  • Utilized time-lapse interference reflection microscopy (IRM) coupled with laser scanning confocal microscopy.

Related Experiment Videos

  • Examined chick retinal ganglion cell growth cones cultured on different substrates.
  • Analyzed images to differentiate areas of close substrate apposition (dark) from distant areas (light).

    • Main Results:

    • Growth cones on laminin and merosin showed minimal substrate contact (uniformly light).
    • Growth cones on N-cadherin exhibited varied contact, with darker filopodial bases and punctate regions.
    • Growth cones on L1 displayed more extensive and darker substrate contact areas compared to other substrates.
    • Observed substrate contact differences correlated with varying growth rates.
    • Growth cone contacts were less dark and stable than fibroblast focal contacts.

    Conclusions:

    • Substrate composition significantly influences growth cone-substrate interactions.
    • N-cadherin and L1 facilitate distinct and more intimate growth cone-substrate contacts than laminin or merosin.
    • These contact differences likely play a role in regulating neuronal growth and migration speed.