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[Construction of gene library of attenuated liver hepatitis A vaccine (H2 strain)]
Zhonghua Yi Xue Za Zhi
|May 1, 1996
Abstract
Objective:
To determine the complete gene library of attenuated live hepatitis A vaccine (H2 strain) from cloned cDNA by application of reverse transcription (RT) and polymerase chain reaction (PCR) technique.
Methods:
Attenuated HAV H2 strain that was selected and studied in China was proven attenuated for human beings and proved to be effective in preventing hepatitis A.
Results:
Ten overlapping cDNA clones were obtained in addition to spanned the entire genome. The restriction enzyme mapping was also determined.
Conclusion:
The gene library will benefit for the sequence of HAV cDNA and study on attenuation.