[Interaction of human serum albumin with berberine hydrochloride by fluorescence method]

Fluorescence method has been used to observe the fluorescence quenching of human serum albumin (HSA) by its interaction with berberine hydrochloride (BH). The interaction dissociation constants of HSA and BH have been determined from a double reciprocal Lineweaver-Burk plot (Kd = 1.73 x 10(5) mol/L).