Related Experiment Videos
Proto-oncogene Sno expression, alternative isoforms and immediate early serum response
S Pearson-White1, R Crittenden
1Department of Microbiology, Box 441, University of Virginia Medical Center, Charlottesville, VA 22908, USA. sp3i@virginia.edu
Nucleic Acids Research
|July 15, 1997
Summary
The study identified and characterized two isoforms of the mouse Sno gene, SnoN and SnoN2. Both isoforms are involved in cellular responses to proliferation stimuli, with SnoN2 showing higher induction.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- The mouse Sno gene, a homolog of the Ski proto-oncogene, produces two isoforms: SnoN and SnoN2.
- SnoN2 differs from SnoN by the absence of a 138 nucleotide coding segment, a result of alternative splicing.
Purpose of the Study:
- To clone and sequence the human ortholog of mouse SnoN2.
- To investigate the expression patterns and regulation of SnoN and SnoN2 isoforms in response to proliferation stimuli.
Main Methods:
- Cloning and sequencing of the human SNON2 gene.
- Analysis of mRNA expression in various mouse and human tissues.
- Serum stimulation of quiescent fibroblasts in the presence of cycloheximide to study gene induction kinetics.
Main Results:
- The human ortholog of mouse SnoN2 was successfully cloned and sequenced.
- Both mouse SnoN and SnoN2 are expressed during embryonic development, in neonatal muscle, and adult tissues, with SnoN2 being predominant in most tissues.
- Expression of both SnoN and SnoN2 mRNAs is rapidly induced by serum stimulation in fibroblasts, independent of new protein synthesis, with SnoN2 induction being significantly higher than SnoN.
- Human SNON2 is expressed at lower levels compared to SNON.
Conclusions:
- The data support a role for Sno isoforms, particularly SnoN2, in mediating cellular responses to proliferation stimuli.
- The differential expression and induction kinetics of SnoN and SnoN2 suggest distinct or complementary functions in cellular processes.