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Multiple bcl-2/Ig gene rearrangements in persistent polyclonal B-cell lymphocytosis
1Centre d'Hématologie et d'Immunologie Clinique, Hôpital du St-Sacrement, Laval University, Quebec, Ste-Foy, Canada.
British Journal of Haematology
|June 1, 1997
Summary
Persistent polyclonal B-cell lymphocytosis, a disorder in women, is linked to bcl-2/Ig gene rearrangement. This finding is crucial for accurate diagnosis, preventing misdiagnosis as non-Hodgkin
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- Persistent polyclonal B-cell lymphocytosis (PPBL) is a benign lymphoproliferative disorder exclusively affecting women.
- PPBL is characterized by binucleated lymphocytes, polyclonal B-cell expansion, and elevated serum IgM.
- The bcl-2 oncogene's role in inhibiting apoptosis suggests a potential link to lymphoproliferative disorders.
Purpose of the Study:
- To investigate the presence of bcl-2/Ig gene rearrangement in patients with persistent polyclonal B-cell lymphocytosis.
- To determine the clinical significance of bcl-2/Ig gene rearrangement in the context of PPBL.
Main Methods:
- Polymerase chain reaction (PCR) was employed to detect bcl-2/Ig gene rearrangement.
- PCR targeted the common breakpoint regions associated with the t(14;18) translocation.
- Peripheral blood mononuclear cells from six PPBL patients were analyzed.
Main Results:
- Bcl-2/Ig gene rearrangement was identified in all six patients studied.
- Multiple bcl-2/Ig gene rearrangements were detected in five out of six patients.
- The estimated frequency of bcl-2/Ig gene rearrangement ranged from 1 in 100 to 1 in 1000 peripheral blood mononuclear cells.
Conclusions:
- Persistent polyclonal B-cell lymphocytosis is associated with bcl-2/Ig gene rearrangement.
- The presence of bcl-2/Ig gene rearrangement in PPBL is clinically significant.
- These findings help differentiate PPBL from leukaemic non-Hodgkin's lymphoma, preventing misdiagnosis.