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Hydrosalpinx fluid enhances human trophoblast viability and function in vitro: implications for embryonic
S W Sawin1, J R Loret de Mola, F Monzon-Bordonaba
1Department of Obstetrics and Gynecology, University of Pennsylvania Medical Center, Philadelphia 19104-4283, USA.
Objective:
To assess the effects of hydrosalpinx fluid on human cytotrophoblast viability and function in vitro.
Design:
Human cytotrophoblasts obtained from third-trimester placentas were cultured in vitro with hydrosalpinx fluid, and cell viability and protein production were assayed.
Setting:
A university hospital.
Patient(S):
Ten hydrosalpinx fluid samples obtained from seven women with clearly diagnosed hydrosalpinges.
Intervention(S):
Recovery of hydrosalpinx fluid by transvaginal aspiration or at the time of surgery.
Main Outcome Measure(S):
Cell viability was assessed by the XTT assay. Secretion of trophoblast oncofetal fibronectin (tropho-uteronectin) and beta-hCG by cultured trophoblasts was determined by Western blot and ELISA of the culture media.
Result(S):
With increasing concentrations of hydrosalpinx fluid from 0% to 20%, there was a significant increase in trophoblast cell viability (1.63-fold increase in 20% hydrosalpinx fluid). Likewise, both Western blot and ELISA assays demonstrated a significant increase in tropho-uteronectin production by trophoblasts with increasing hydrosalpinx fluid concentrations (3.76-fold increase in 20% hydrosalpinx fluid). beta-Human chorionic gonadotropin production also increased significantly in the presence of hydrosalpinx fluid (3.31-fold increase in 20% hydrosalpinx fluid).
Conclusion(S):
These findings suggest that hydrosalpinx fluid improves human trophoblast viability in vitro and enhances the production of tropho-uteronectin and beta-hCG by these cells.