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Related Experiment Videos

Localization of nerve cells in the developing rat tooth

K Luukko1, K Sainio, H Sariola

  • 1Programme of Development Biology, University of Helsinki, Finland.

Journal of Dental Research
|July 1, 1997
PubMed
Summary

Neurons are present in developing mammalian teeth, challenging previous beliefs that they are unnecessary for tooth formation. This study found neuronal cell bodies and axons associated with tooth germs, suggesting a potential role in regulating tooth development.

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Area of Science:

  • Developmental Biology
  • Neuroscience
  • Oral Biology

Background:

  • Previous research indicated mammalian tooth formation occurs without peripheral nerve fibers, suggesting neurons are not essential for development.
  • Recent findings of peripherin, a neuronal marker, near developing teeth suggested potential neuronal involvement.

Purpose of the Study:

  • To investigate the presence and association of neuronal cells within developing rat tooth germs.
  • To determine if neuronal cells play a role in mammalian tooth formation.

Main Methods:

  • In vitro culture of embryonic rat first molars (E14, E16) to observe neuronal presence.
  • Immunohistochemistry using antibodies for peripherin and L1 neural cell adhesion protein.
  • In situ hybridization to detect neurofilament light-chain (NF-L) mRNA expression in developing tooth germs (E12-E15).

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Main Results:

  • Peripheral trigeminal axons degenerated in vitro, but neuronal cell bodies and axons were detected using specific antibodies.
  • NF-L mRNA expression was observed in developing tooth germs starting at the bud stage (E15), located adjacent to the dental epithelium.
  • NF-L expression patterns correlated with peripherin-positive cell localization, indicating the presence of neuronal cells.

Conclusions:

  • Neuronal cells, not just peripheral nerve projections, are associated with developing mammalian teeth.
  • The presence of neuronal cells suggests they may actively participate in regulating tooth formation processes.