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Related Experiment Videos

Possible origin of murine AIDS-inducing sequence

Y Kubo1, K Higo, H Kobayashi

  • 1Department of Viral Oncology, Kyoto University, Japan.

Leukemia
|April 1, 1997
PubMed
Summary

Murine AIDS (MAIDS) virus evolution involved unique gag p12 region mutations. Frameshift mutations during recombination between helper virus and Edv transcript likely generated the pathogenic MAIDS virus.

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Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Murine AIDS (MAIDS) is caused by a unique virus with a distinct gag p12 region sequence.
  • A related transcript, Edv, is expressed in normal C57BL/6 mice and proposed as the MAIDS virus origin.
  • MAIDS virus was initially isolated from radiation-induced leukemic C57BL/6 mice.

Purpose of the Study:

  • To investigate the genetic basis of the MAIDS virus.
  • To compare the gag p12 sequences of the MAIDS virus, helper virus, and Edv transcript.
  • To elucidate the mechanism of MAIDS virus generation.

Main Methods:

  • Molecular cloning and sequencing of the Edv transcript.
  • Nucleotide sequence comparison between MAIDS virus, helper LP-BM5 ecotropic virus, and Edv transcript.

Related Experiment Videos

  • Analysis of deletions, insertions, and frameshift mutations in the gag p12 region.

    • Main Results:

    • The pathogenic MAIDS virus has 16-bp deletions and a 1-bp insertion in its gag p12 region compared to the helper virus.
    • The Edv transcript contains only a 3-bp deletion in the homologous region.
    • Frameshift mutations in the gag p12 region of MAIDS virus resulted in reduced amino acid sequence homology.

    Conclusions:

    • MAIDS virus likely originated from recombination between a helper virus and the Edv transcript or a similar sequence.
    • Frameshift mutations in the gag p12 region are crucial for the generation of the pathogenic MAIDS virus.
    • Understanding these genetic alterations provides insights into retroviral evolution and pathogenesis.