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Epidermal growth factor and temperature regulate keratinocyte differentiation

M Ponec1, S Gibbs, A Weerheim

  • 1Department of Dermatology, Leiden University Hospital, The Netherlands.

Archives of Dermatological Research
|May 1, 1997
PubMed
Summary
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Optimizing skin culture models requires understanding temperature and epidermal growth factor (EGF) effects. Lower temperatures (33°C) without EGF promote native-like epidermal differentiation, while EGF at 37°C increases thickness but impairs differentiation.

Area of Science:

  • Tissue engineering
  • Dermatology
  • Cell biology

Background:

  • Current skin culture models have limitations in lifespan and barrier function, hindering pharmacological and toxicological applications.
  • Optimization of culture conditions is crucial for developing more accurate in vitro skin models.

Purpose of the Study:

  • To investigate the impact of temperature and epidermal growth factor (EGF) on epidermal differentiation and lipogenesis in reconstructed epidermis.
  • To evaluate the potential of modified culture conditions for improving skin models.

Main Methods:

  • Reconstructed epidermis on de-epidermized dermis was cultured at 37°C and 33°C, with and without EGF.
  • Epidermal differentiation was assessed by analyzing protein markers (keratins 1 & 10, involucrin, transglutaminase) and lipid markers (ceramides).

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  • Morphological changes and lipid droplet accumulation were examined.

    • Main Results:

    • At 37°C, EGF increased epidermal thickness, particularly the stratum corneum, and triglyceride content, suggesting hyperproliferation.
    • At 33°C without EGF, epidermis showed morphology closer to native skin, with reduced stratum corneum thickness and normal lipid levels.
    • EGF at 33°C impaired epidermal morphogenesis, suppressed keratin synthesis (early differentiation marker), but minimally affected ceramide synthesis (late differentiation marker).

    Conclusions:

    • Epidermal growth factor (EGF) significantly impacts keratin synthesis, which is sensitive to both temperature and EGF presence.
    • Ceramide synthesis is less affected by EGF and temperature, indicating differential regulation of differentiation markers.
    • Lower temperatures (33°C) without EGF yield superior epidermal differentiation for skin culture models.