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Genomic regions of coxsackievirus B3 associated with cardiovirulence

C Lee1, E Maull, N Chapman

  • 1Department of Microbiology, University of Arkansas for Medical Science, Little Rock, USA.

Journal of Medical Virology
|July 1, 1997
PubMed

Insights

The 5' nontranslated region (NTR) of coxsackievirus B3 (CVB3) is the primary determinant of cardiovirulence. Specific capsid proteins also contribute to CVB3

Area of Science:

  • Virology
  • Molecular Biology
  • Cardiology

Background:

  • Coxsackievirus B3 (CVB3) is a significant cause of viral myocarditis.
  • Understanding the genetic basis of CVB3 cardiovirulence is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To elucidate the molecular determinants of cardiovirulence within the CVB3 genome.
  • To identify specific viral genomic regions responsible for CVB3-induced myocarditis.

Main Methods:

  • Construction and analysis of infectious chimeric cDNAs from virulent (CVB3m) and non-virulent (CVB30) CVB3 strains.
  • Assessment of cardiovirulence in a murine model of acute myocarditis.
  • Comparative analysis of nucleotide and amino acid sequences in key viral genomic regions.

Main Results:

  • The 5' nontranslated region (5' NTR) of the CVB3 genome plays a major role in determining cardiovirulence.
  • The genomic region encoding capsid proteins contributes a minor, additive effect to cardiovirulence.
  • Sequence variations within the 5' NTR, particularly in stem-loop motifs, and specific amino acid changes in VP2 and VP3 are implicated in cardiovirulence.

Conclusions:

  • Cardiovirulence in CVB3 is a multifactorial trait influenced by both the 5' NTR and capsid protein-encoding regions.
  • Specific sequence elements within the 5' NTR and capsid proteins are critical for CVB3's ability to cause myocarditis.

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