Related Experiment Videos
Cloning and nucleotide sequence of Bacillus stearothermophilus pyruvate carboxylase
1Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Japan. kondo@bse.kyutech.ac.jp
Gene
|May 20, 1997
Abstract:
A gene for prokaryotic pyruvate carboxylase (PC) was cloned from Bacillus stearothermophilus. It has an open reading frame of 3441 base pairs which can code for a protein of 128,353 Da. Not only the molecular size and domain organization but also the deduced amino acid sequence of B. stearothermophilus PC are similar to those of eukaryotic PCs.