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Molecular analysis of katG gene mutations in strains of Mycobacterium tuberculosis complex from Africa
1Department of General Pediatrics, Children's Hospital, Heidelberg University, Germany. ic1@aixterm1.urz.uni-heidelberg.de
Antimicrobial Agents and Chemotherapy
|July 1, 1997
Abstract:
A sample of 124 isoniazid (INH)-resistant and 88 susceptible strains of Mycobacterium tuberculosis complex from south, central, and west Africa was analyzed by direct sequence analysis and PCR-restriction fragment length polymorphism analysis of their catalase-peroxidase (katG) genes. Point mutations at codon 315 were found in the genomes of 64% of INH-resistant strains, but no complete deletions were identified. Mutations at codon 463 were independent of INH resistance and were linked to the geographic origins of the strains.