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Mapping quantitative trait loci for open-field behavior in mice

H K Gershenfeld1, P E Neumann, C Mathis

  • 1Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas 75235-8898, USA. hgersh@mednet.swmed.edu

Behavior Genetics
|May 1, 1997
PubMed
Summary

This study mapped quantitative trait loci (QTL) for mouse open field (O-F) behavior, identifying genetic regions on chromosomes 1, 3, 10, and 19 influencing novelty response and habituation. These findings help understand the genetic basis of individual behavioral differences.

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Area of Science:

  • Behavioral Genetics
  • Neuroscience
  • Animal Models

Background:

  • Individual differences in behavioral responses, such as to a novel environment, are complex.
  • The open field (O-F) test is a standard method for assessing exploratory behavior and anxiety-like phenotypes in rodents.
  • Understanding the genetic architecture of these behaviors is crucial for dissecting their underlying mechanisms.

Purpose of the Study:

  • To identify quantitative trait loci (QTL) associated with open field (O-F) behavior in mice.
  • To map genetic regions influencing both initial responses to novelty and habituated behavior in the O-F.
  • To provide a genetic framework for further investigation into the biological basis of behavioral variation.

Main Methods:

  • Whole genome quantitative trait loci (QTL) mapping was performed using an F2 intercross between A/J and C57BL/6J mouse strains.

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  • Behavioral phenotyping included measurements of ambulation and vertical rearings in the open field (O-F) during initial exposure and after habituation.
  • Statistical analysis involved calculating logarithm of the odds (lod) scores to identify significant QTL.

    • Main Results:

    • Significant QTL influencing initial ambulation and rearing were identified on chromosomes 1 and 10.
    • QTL affecting habituated ambulation were found on chromosomes 3 and 10, while habituated rearing QTL were on chromosomes 1, 10, and 19.
    • A specific QTL on chromosome 1 influenced initial ambulation, whereas a QTL on chromosome 10 affected both initial and habituated rearing.
    • Collectively, identified QTL explained 16.3% to 24.1% of the phenotypic variance in O-F behaviors.

    Conclusions:

    • Multiple independent QTL significantly contribute to the complex behavioral phenotype observed in the open field (O-F) test.
    • These QTL provide valuable genetic markers for dissecting the neurobiological underpinnings of behavioral responses to novelty and habituation.
    • The identified genetic loci offer a foundation for future research into the genetic architecture of individual differences in behavior.