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Updated: Aug 11, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Gas chromatographic/mass spectrometric measurement of ascorbic acid and analysis of ascorbic acid degradation in
1Department of Medicine, University of Colorado Health Sciences Center, Denver 80220, USA.
Abstract:
L-Ascorbic acid, DHA, and the oxidized products derived from AA can be accurately measured using GC/MS. Owing to the complex nature of the reactions through which AA proceeds, we believe that GC/MS is currently the procedure of choice in making AA-related measurements. The methods described are useful in defining reactions involving AA. The methods may indicate in vivo oxidative injury and may allow the use of AA-derived products to determine if antioxidant modulations are effective.
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