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PCR-mediated detection of acidophilic, bioleaching-associated bacteria
P De Wulf-Durand1, L J Bryant, L I Sly
1Department of Microbiology, University of Queensland, Brisbane, Australia.
Applied and Environmental Microbiology
|July 1, 1997
Abstract:
The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg.
