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A MALDI probe for mass spectrometers

J D Lennon1, G L Glish

  • 1Department of Chemistry, University of North Carolina at Chapel Hill 27599-3290, USA.

Analytical Chemistry
|July 1, 1997
PubMed
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A novel Matrix-Assisted Laser Desorption/Ionization (MALDI) probe utilizes transmission geometry, enabling its use in confined ion sources like quadrupole ion traps. This versatile probe facilitates MALDI analysis across various mass spectrometers with minimal equipment.

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Matrix-Assisted Laser Desorption/Ionization (MALDI) is a crucial technique in mass spectrometry.
  • Traditional MALDI methods often require specific instrument configurations and space.
  • Spatially constrained ion sources, such as those in quadrupole ion trap mass spectrometers, present challenges for sample introduction.

Purpose of the Study:

  • To design and demonstrate a novel MALDI probe utilizing transmission geometry.
  • To enable MALDI analysis in spatially constrained ion sources, particularly quadrupole ion traps.
  • To assess the performance and versatility of the new probe design.

Main Methods:

  • Development of a MALDI probe with transmission geometry, allowing sample irradiation from the backside via fiber optics.

Related Experiment Videos

  • Direct deposition of samples onto a quartz substrate for analysis.
  • Integration and testing of the probe with a quadrupole ion trap mass spectrometer.

    • Main Results:

    • The probe successfully performed MALDI on a quadrupole ion trap mass spectrometer, yielding comparable results to traditional methods.
    • Sensitive detection was achieved, with spectra obtained from 50 femtomoles (fmol) of bombesin.
    • Tandem mass spectrometry (MS/MS) was performed on 5 picomoles (pmol) of des-Arg9-bradykinin.
    • Analyte ion signal stability was demonstrated, lasting over 2500 laser shots for 2 pmol of bombesin.
    • Optical micrographs revealed sample crystal distribution as a function of laser shots.

    Conclusions:

    • The developed transmission geometry MALDI probe is effective for use with quadrupole ion trap mass spectrometers.
    • The probe design offers similar performance to conventional MALDI techniques.
    • Its adaptability allows for MALDI analysis on multiple mass spectrometry instruments using a single laser and fiber optic setup.