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Thiabendazole-induced cytogenetic abnormalities in mouse oocytes
J B Mailhes1, D Young, M J Aardema
1Department of Obstetrics and Gynecology, Louisiana State University Medical Center, Shreveport 71130, USA.
Environmental and Molecular Mutagenesis
|January 1, 1997
Summary
Thiabendazole (TBZ) can cause a small but significant increase in aneuploid oocytes, a type of genetic disorder. This chemical exposure also negatively impacts ovulation in female mice.
Area of Science:
- Reproductive toxicology
- Human genetics
- Developmental biology
Background:
- Aneuploidy is a common human genetic disorder, often linked to maternal age, but its chemical causes, especially in germ cells, are poorly understood.
- Thiabendazole (TBZ) has conflicting data regarding its potential to induce aneuploidy, necessitating further investigation into its effects on oocytes.
Purpose of the Study:
- To investigate the potential of thiabendazole (TBZ) to induce aneuploidy in mouse oocytes.
- To assess the impact of TBZ exposure on ovulation and oocyte collection in female mice.
Main Methods:
- Female ICR mice were administered varying doses of TBZ (0, 50, 100, 150 mg/kg) via intraperitoneal injection.
- Oocytes were collected and analyzed for hyperploidy (aneuploidy).
- Ovulatory success and oocyte yield per mouse were recorded.
Main Results:
- A statistically significant increase in hyperploid oocytes was observed at the 100 mg/kg TBZ dose compared to controls.
- TBZ exposure led to reduced proportions of ovulatory mice and fewer oocytes collected per ovulatory female at tested doses.
Conclusions:
- Thiabendazole (TBZ) induces a statistically significant, albeit small, increase in aneuploid oocytes at toxic doses.
- These toxic TBZ doses also impair ovulation, indicating potential reproductive health risks.