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Human endothelial culture supernatant selectively promotes IgM-producing hybridomas
R Greferath1, S Sauer-Nehls, S Dyballa
1Center for Blood Research Laboratories, Harvard Medical School, Boston, MA 02135, USA.
Journal of Immunological Methods
|May 26, 1997
Abstract:
We compared the effect of human endothelial cell culture supernatant (HECS), ESG (Ewing sarcoma growth factor), IL-6 (interleukin-6) and peritoneal macrophages on the recovery of hybridoma cells after fusion with respect to growth, stability and distribution of isotype variants. A selective growth of murine IgM-producing hybridoma cells was observed in the presence of HECS after cell fusion.