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[Contribution of flow cytometry to allergologic diagnosis]
A Sabbah1, M Drouet, J Sainte-Laudry
1Laboratoire d'Immuno-Allergologie-CHU Angers.
Allergie Et Immunologie
|January 1, 1997
Summary
Flow Cytometry for activation of basophils (TAB) effectively diagnoses allergies. This method, using CD63 marker, shows greater accuracy than total IgE for identifying atopy and drug sensitivities.
Area of Science:
- Immunology
- Allergology
- Cellular Biology
Background:
- Drug allergy diagnosis remains a challenge.
- Current methods like total IgE and skin tests have limitations.
- Flow Cytometry for activation of basophils (TAB) is an emerging research pathway.
Purpose of the Study:
- To evaluate the utility of TAB in diagnosing allergies.
- To compare TAB's efficacy against total IgE for atopy assessment.
- To investigate TAB's role in drug allergy diagnosis, specifically for Myorelaxants.
Main Methods:
- Utilized Flow Cytometry for activation of basophils (TAB) assay.
- Measured CD63 expression as a marker for basophil activation.
- Included studies on Pneumoallergens, Hymenoptera venoms, and drug-allergic patients.
Main Results:
- TAB demonstrated enhanced basophil fluorescence in atopic subjects compared to non-atopic individuals.
- TAB proved more effective than total IgE in defining subject atopy.
- TAB showed significant utility in diagnosing allergies to specific drugs, including Myorelaxants.
Conclusions:
- Flow Cytometry for activation of basophils (TAB) is a valuable tool for allergy diagnosis.
- TAB offers improved atopy definition and drug allergy detection.
- Further research into TAB applications in clinical immunology is warranted.