Effects of aging and diet on the accumulation of dolichol in rat tissues

A diet containing 15% (w/w) fat and 20% (w/w) of either casein or soy protein was fed to rats between 1 and 18 months of age. The effects of these dietary proteins on the accumulation of cholesterol and dolichols in kidney, spleen, brain, and heart were studied. The amount of cholesterol in these tissues was not influenced by the diet. In kidney and spleen, the amount of dolichols in rats fed the experimental diets was 50-70% higher than those in rats fed the lab chow diet. The contents of spleen dolichols in rats fed the soy protein diet tended to be higher than those in rats fed the casein diet. The amount of dolichols in heart and brain was not influenced by the diet. The proportion of spleen dolichyl fatty ester in rats fed the experimental diets was higher than that in rats fed the lab chow. The distribution of the dolichol isoprenologues was not influenced by the diet. There was a shift in the dolichol isoprenologues in kidney and spleen toward ones of lower chain length until 2 months of age, and after that there was no change. However, in heart and brain they shifted toward ones of lower chain length with aging. Our results suggested that dolichol metabolism may be influenced by fat content in the diets and differed among rat tissues.