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[A new embedding material for morphometry: Shiojirin E-10]

M Shibata1, M Suzuki, N Goto

  • 1Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.

Kaibogaku Zasshi. Journal of Anatomy
|December 1, 1996
PubMed
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Shiojirin E-10, a nitrocellulose ether alcohol solution, effectively substitutes for Celloidin in morphometric studies. This new embedding material shows no significant difference in tissue shrinkage or staining characteristics for nerve cell and axon measurements.

Area of Science:

  • Neuroscience
  • Histology
  • Biomaterials

Background:

  • Celloidin embedding is crucial for morphometric studies using Luxol fast blue-PAS-Hematoxylin (LPH) staining.
  • The suspension of Celloidin manufacture in Japan necessitates an alternative embedding material.

Purpose of the Study:

  • To evaluate Shiojirin E-10 as a substitute for Celloidin in morphometric studies.
  • To compare tissue shrinkage and staining characteristics between Celloidin and Shiojirin E-10.

Main Methods:

  • Secondary fixation with chromic acid and embedding in either Celloidin or Shiojirin E-10.
  • LPH staining of human cauda equina sections for axonal area measurement using an image analyzer.
  • Hue analysis of H-E stained mouse liver/kidney and LPH stained human cauda equina sections.

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Main Results:

  • No significant difference in axonal area measurements between Celloidin (4.31 ± 2.55 μm²) and Shiojirin E-10 (4.14 ± 2.20 μm²) sections.
  • Comparable hue values for hematoxylin, eosin, axonal, and myelin sheath staining between the two embedding materials.
  • Shiojirin E-10 demonstrated similar qualitative and quantitative staining characteristics to Celloidin.

Conclusions:

  • Shiojirin E-10 is a suitable substitute for Celloidin in morphometric studies.
  • The use of Shiojirin E-10 maintains the integrity of histological staining and quantitative measurements.
  • This finding addresses the material shortage and ensures continuity in neuroanatomical research.