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Updated: Aug 15, 2026

A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii
09:17

A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii

Published on: January 2, 2017

[Enhancement of treatment efficacy in experimental plague by the sequential administration of antibiotics]

L N Makarovskaia, L A Tinker, E I Markovskaia

    Antibiotiki I Khimioterapiia = Antibiotics and Chemoterapy [Sic]
    |January 1, 1996
    PubMed

    Insights

    Combining rifampicin with ampicillin, azlocillin, or polymyxin significantly improved plague survival in mice. Delayed administration and reduced frequency of these antibiotics further enhanced survival rates compared to rifampicin alone.

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Pharmacology

    Background:

    • Plague remains a significant public health concern, necessitating effective therapeutic strategies.
    • Rifampicin monotherapy at sub-optimal doses offers limited protection against plague.
    • Combination therapy may overcome antibiotic resistance and improve treatment outcomes.

    Purpose of the Study:

    • To evaluate the efficacy of combination antibiotic therapy for plague treatment.
    • To assess the impact of delayed administration and reduced dosing frequency on survival rates.

    Main Methods:

    • Murine model of plague infection.
    • Administration of rifampicin alone or in combination with ampicillin, azlocillin, or polymyxin.
    • Varying the timing and frequency of antibiotic administration.

    Main Results:

    • Combination therapy with ampicillin, azlocillin, or polymyxin, initiated 24 or 96 hours post-infection alongside daily rifampicin, resulted in 80-100% animal survival.
    • Sequential administration of ampicillin, azlocillin, or polymyxin with rifampicin led to a threefold increase in survival compared to rifampicin alone.
    • Decreased administration frequency and increased intervals between doses of the combination drugs significantly improved survival rates.

    Conclusions:

    • Combination antibiotic therapy demonstrates superior efficacy in treating plague compared to rifampicin monotherapy.
    • Optimizing the dosing schedule of combination therapy can further enhance survival outcomes in plague treatment.

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