Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple exocytotic pathways in pancreatic beta cells

N Takahashi1, T Kadowaki, Y Yazaki

  • 1Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.

The Journal of Cell Biology
|July 14, 1997
PubMed
Summary

Mouse pancreatic beta cells utilize distinct calcium-dependent exocytotic pathways. Researchers identified three pathways with different fusion speeds, impacting serotonin secretion and vesicle recycling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Eight-year survival with metastatic oesophageal carcinoma: a case report and review of the literature.

South African journal of surgery. Suid-Afrikaanse tydskrif vir chirurgie·2026
Same author

Artifact reduction in low and ultra-low dose chest computed tomography for patients with pacemaker: A phantom study.

Radiography (London, England : 1995)·2024
Same author

The effect of nano hydroxyapatite coating implant surfaces on gene expression and osseointegration.

Medicina oral, patologia oral y cirugia bucal·2023
Same author

Visualization of Nigrosome 1 from the Viewpoint of Anatomic Structure.

AJNR. American journal of neuroradiology·2019
Same author

Sustained rescue of prefrontal circuit dysfunction by antidepressant-induced spine formation.

Science (New York, N.Y.)·2019
Same author

Efficacy and safety of trastuzumab, lapatinib, and paclitaxel neoadjuvant treatment with or without prolonged exposure to anti-HER2 therapy, and with or without hormone therapy for HER2-positive primary breast cancer: a randomised, five-arm, multicentre, open-label phase II trial.

Breast cancer (Tokyo, Japan)·2018

Area of Science:

  • Cell Biology
  • Endocrinology
  • Neuroscience

Background:

  • Pancreatic beta cells regulate glucose homeostasis via regulated exocytosis.
  • Calcium (Ca2+) influx is a primary trigger for exocytosis in beta cells.
  • Understanding exocytotic pathways is crucial for metabolic disease research.

Purpose of the Study:

  • To investigate Ca2+-dependent exocytotic pathways in mouse pancreatic beta cells.
  • To characterize the kinetics and characteristics of different exocytosis modes.
  • To elucidate the relationship between exocytosis and endocytosis in beta cells.

Main Methods:

  • Capacitance measurement to monitor membrane fusion during exocytosis.
  • Amperometric detection of serotonin secretion from large dense-core vesicles.

Related Experiment Videos

  • Induction of exocytosis using caged-Ca2+ compounds to control cytosolic Ca2+ levels.

    • Main Results:

    • Two major exocytosis components were identified, with only the slow component linked to serotonin secretion.
    • Fast and slow exocytoses were associated with distinct endocytosis mechanisms.
    • Two types of quantal event responses (type-1 and type-2) with different delays and durations were observed.

    Conclusions:

    • Mouse pancreatic beta cells possess at least three exocytotic pathways with distinct fusion kinetics.
    • These pathways differ in their temporal dynamics and vesicle secretion patterns.
    • Findings provide a new framework for understanding beta cell function and dysfunction.