Related Experiment Video
Updated: Aug 11, 2026
10:23
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Published on: December 4, 2017
Determination of endogenous peptides with in vitro ACE inhibitory activity in normotensive human plasma by the
T Matsui1, H Matsufuji, T Kawasaki
1Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Bioscience, Biotechnology, and Biochemistry
|June 1, 1997
Abstract:
An in vitro degradation test of angiotensin (ANG) II or III in normotensive supine human plasma from 9 healthy male subjects confirmed the production of smaller ANG metabolites with angiotensin I-converting enzyme inhibitory activity. These metabolites were identified as ANG (3-8), ANG (5-8), and ANG (3-4), whose respective peptide concentrations were determined by our proposed naphthalene-2,3-dialdehyde (NDA)-HPLC method to be 64 +/- 9, 39 +/- 5, 176 +/- 22, and 197 +/- 35 fmol/ml of plasma.
