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Related Experiment Videos

Prostate-specific membrane antigen

W R Fair1, R S Israeli, W D Heston

  • 1Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.

The Prostate
|July 1, 1997
PubMed
Summary
This summary is machine-generated.

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Researchers elucidated the prostate-specific membrane antigen (PSM), a novel prostate antigen. PSM serves as a valuable marker for detecting metastatic prostate cancer spread and a potential target for imaging and therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Investigating novel prostate-specific antigens (PSAs) is crucial for understanding prostate function and tumor progression.
  • Elucidation of the structure and function of the prostate-specific membrane antigen (PSM) contributes to this understanding.

Purpose of the Study:

  • To characterize the prostate-specific membrane antigen (PSM).
  • To evaluate PSM's potential as a diagnostic marker and therapeutic target in prostate cancer.

Main Methods:

  • Utilized the 7E11-C5.3 monoclonal antibody for PSM recognition.
  • Isolated PSM cDNA via PCR using degenerate primers derived from tryptic peptides.

Main Results:

  • Identified PSM as a 100 kD glycoprotein, a type II integral membrane protein.

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  • Extensive characterization of the PSM protein and its cDNA was performed.

    • Conclusions:

    • PSM is a valuable marker for detecting hematogenous micrometastatic tumor dissemination via RT-PCR assays.
    • PSM exhibits properties suitable for molecular targeting in antibody-based imaging and therapeutic strategies for prostate cancer.