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Toxinogenicity of heat-resistant fungi detected by a bio-assay

E Piecková1, Z Jesenská

  • 1Institute of Preventive and Clinical Medicine, Bratislava, Slovakia.

International Journal of Food Microbiology
|May 20, 1997
PubMed

Insights

This study evaluated fungal metabolites for ciliostatic activity, finding that Dichotomomyces cejpii and Eupenicillium baarnense strains exhibited significant effects on tracheal cilia. Other fungal isolates showed varying levels of metabolite production and activity.

Area of Science:

  • Mycology
  • Microbiology
  • Biochemistry

Background:

  • Fungal secondary metabolites possess diverse biological activities.
  • Ciliostatic compounds can impact respiratory tract health.
  • Heat-resistant fungi are a source of novel bioactive compounds.

Purpose of the Study:

  • To investigate the ciliostatic activity of exo- and endometabolites from heat-resistant fungal isolates.
  • To identify fungal strains producing metabolites that inhibit ciliary function.
  • To determine the localization (medium, mycelium, spores) of these ciliostatic metabolites.

Main Methods:

  • In vitro bioassay using tracheal tissue cultures from 1-day-old chicks.
  • Evaluation of 125 heat-resistant fungal isolates.
  • Extraction and detection of chloroform-soluble secondary metabolites.

Main Results:

  • Eleven percent of isolates produced detectable chloroform-extractable metabolites in the medium.
  • Twenty-three percent yielded metabolites from mycelium and spores.
  • Twenty percent had metabolites in all three fractions (medium, mycelium, spores).
  • Dichotomomyces cejpii and Eupenicillium baarnense strains demonstrated notable ciliostatic activity.
  • Gilmaniella humicola ATCC 96467 produced ciliostatic metabolites in medium, mycelia, and spores.

Conclusions:

  • Specific fungal strains, notably Dichotomomyces cejpii and Eupenicillium baarnense, possess significant ciliostatic potential.
  • Metabolite localization varies among fungal species, with some producing active compounds across multiple fractions.
  • Further research into these fungal metabolites could yield novel therapeutic agents for respiratory conditions.

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