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High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

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A cryptic DNA binding domain at the COOH terminus of TFIIIB70 affects formation, stability, and function of

J Huet1, C Conesa, C Carles

  • 1Service de Biochimie et Génétique Moléculaire, Commissariat à l'Energie Atomique, Saclay, F91191 Gif sur Yvette Cedex, France.

The Journal of Biological Chemistry
|July 18, 1997
PubMed

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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