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Related Experiment Videos

Immunocytochemistry controls using cell culture

D F Kurtycz1, R Logrono, M Leopando

  • 1Department of Pathology, University of Wisconsin, Madison 53706, USA. dkurtycz@facstaff.wisc.edu

Diagnostic Cytopathology
|July 1, 1997
PubMed
Summary
This summary is machine-generated.

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Cytology labs face challenges with control materials for immunocytochemical testing. Cultured anaplastic cells offer a superior method for creating consistent, high-quality control slides with preserved cellular detail.

Area of Science:

  • Cytopathology
  • Immunocytochemistry
  • Cell Biology

Background:

  • Lack of adequate control material is a persistent issue in cytology laboratories for immunocytochemical testing.
  • Current control procedures use paraffin-embedded materials, which differ significantly from cytologic preparations.
  • This discrepancy impacts the reliability and accuracy of immunocytochemical analyses.

Purpose of the Study:

  • To develop an improved method for generating control preparations for immunocytochemical analysis.
  • To utilize cultured anaplastic cells with known antigenic features for creating standardized control slides.
  • To enhance the quality and interpretability of immunocytochemical testing in cytology.

Main Methods:

  • Established control preparations using commercially sourced cultured anaplastic cell lines (melanoma, rhabdomyosarcoma, T-cell leukemia, squamous-cell carcinoma).

Related Experiment Videos

  • Employed modified Saccomano fixation and acetone fixation techniques combined with the cytospin method for slide preparation.
  • Tested cell lines with specific antibodies (HMB-45, actin, leukocyte common antigen (LCA), cytokeratin) using avidin-biotin immunoperoxidase and diaminobenzidine (DAB) chromogens.

    • Main Results:

    • Achieved good-quality cytologic preparations from cultured cell lines.
    • Demonstrated excellent morphologic preservation of cellular detail in the final preparations.
    • Confirmed interpretability of the prepared slides using standard immunocytochemical staining methods.

    Conclusions:

    • Cultured cells provide a superior alternative to traditional control materials for immunocytochemistry.
    • This method allows for the preparation of a virtually unlimited supply of consistent control slides.
    • Laboratories with tissue culture facilities can benefit from this approach to improve immunocytochemical testing accuracy.