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E-selectin is upregulated in proliferating endothelial cells in vitro
J Bischoff1, C Brasel, B Kräling
1Surgical Research Laboratory, Children's Hospital, Boston, MA 02115, USA.
Microcirculation (New York, N.Y. : 1994)
|June 1, 1997
Summary
E-selectin expression increases in proliferating endothelial cells, particularly during the G2/M cell cycle phases. This finding offers insights into endothelial cell behavior during growth and potential roles in angiogenesis.
Area of Science:
- Cell Biology
- Molecular Biology
- Immunology
Background:
- E-selectin is an endothelial cell glycoprotein involved in leukocyte adhesion and angiogenesis.
- Understanding E-selectin's role in cell proliferation and the cell cycle is crucial for endothelial cell biology.
Purpose of the Study:
- To investigate E-selectin polypeptide expression in proliferating versus quiescent bovine capillary endothelial cells.
- To determine the relationship between E-selectin expression and the cell cycle phases.
Main Methods:
- Immunoadsorption and enzyme-linked immunosorbent assay (ELISA) for E-selectin polypeptide analysis.
- Fluorescence-activated cell sorting (FACS) to assess E-selectin expression.
- Propidium iodide staining to analyze endothelial cell cycle distribution (G0/G1, S, G2/M).
Main Results:
- E-selectin was significantly upregulated in proliferating endothelial cells compared to quiescent cells.
- Upregulation of E-selectin was independent of inflammatory activation.
- E-selectin-positive cells showed a higher proportion in the G2 and M phases of the cell cycle compared to E-selectin-negative cells.
Conclusions:
- Increased E-selectin expression correlates with endothelial cell proliferation and the G2/M cell cycle phases under non-inflammatory conditions.
- In vitro findings support the in vivo presence of E-selectin in proliferating endothelial cells.