Efficient preparation of single-stranded DNA for in vitro selection
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1Hans-Knöll-Institut für Naturstoff-Forschung, Abt.Zell- und Molekularbiologie, Jena, Germany.
Molecular Biotechnology
|June 1, 1997
Abstract:
In vitro selection of aptamers requires the reliable enzymatic preparation of large amounts of (+) single-stranded DNA molecules. This can be achieved by selective enzymatic digest of 5'-phosphorylated (-) strands from PCR products, a method already widely used in sequencing of PCR products. Here we present an adaptation of this method to prepare large pools of single-stranded DNA molecules for in vitro selection.
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