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Labeling proteins at high specific activity using N-succinimidyl 4-[18F](fluoromethyl) benzoate
1Positron Emission Tomography Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.
Abstract:
High effective specific activity N-succinimidyl 4-[18F](fluoromethyl)benzoate was prepared using a reversed phase HPLC procedure. Reversed phase HPLC removed several additional impurities not removed by normal phase HPLC, thereby increasing the effective specific activity. Small amount (< 100 micrograms) of sensitive proteins such as erythropoietin can be labeled with this reagent in high yield without aggregation.